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The Role of Steroids, Growth Factors and CAMP Stimulators on the Gap Junction Activity in Cumulus Oocyte Complexes in the Rat

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posted on 2021-11-10, 18:38 authored by Yadav, Manvi

Bidirectional communication between mammalian oocytes and their surrounding somatic cells is essential for oocyte maturation. Gap junctions promote the transfer of essential metabolites, nucleotides, amino acids and ions from cumulus cells to the oocyte that are crucial for oocyte growth and development. However, the range of factors present in the microenvironment of the developing antral follicle, which modulate gap junction activity of the cumulus-oocyte complexes (COCs), is largely unknown. The primary objective of this study was to determine the effects of various steroids, growth factors and cAMP stimulators on the gap junction activity in rat COCs. The gap junction activity was measured in presence or absence of different treatments using a fluorescence dye and in the presence of milrinone, a phosphodiesterase type 3 inhibitor. The major findings of this study were that cAMP stimulators increased the rate of dye transfer from cumulus cells to the oocyte. Under in vitro conditions it was established that neither steroids nor IGF1 by themselves were able to modulate gap junction activity in rat COCs. Furthermore, forskolin, a potent cAMP stimulator; caused a relative increase in Cx37 gene expression levels following a four hours incubation period. The outcomes from the present study may help to provide new insights into developing suitable in vitro conditions, for the in vitro maturation of mammalian oocytes.

History

Copyright Date

2010-01-01

Date of Award

2010-01-01

Publisher

Te Herenga Waka—Victoria University of Wellington

Rights License

Author Retains Copyright

Degree Discipline

Biotechnology

Degree Grantor

Te Herenga Waka—Victoria University of Wellington

Degree Level

Masters

Degree Name

Master of Science

Victoria University of Wellington Item Type

Awarded Research Masters Thesis

Language

en_NZ

Victoria University of Wellington School

School of Biological Sciences

Advisors

McNatty, Ken